Anti-payload Antibodies 제품을 소개합니다.

Antibody-Drug Conjugate (ADC)는 linker를 통해 소분자 세포독성 약물(payload)을 monoclonal antibody에 연결하여 암세포에 세포독성 약물을 정확하게 전달함으로써 종양을 사멸시키는 데 사용됩니다. 일반적으로 사용되는 ADC payload에는 MMAE, MMAF, DM1, DXd 등이 있습니다. GenScript는 ADC 약물동태학 연구를 지원하기 위해 다양한 anti-payload antibody, anti-idiotypic antibody, anti-human IgG Fc antibody 및 재조합 표적 항원을 제공합니다.

[Product Highlights]

[ADC PK Study]

약물동태학은 약물의 흡수, 분포, 대사 및 배설(ADME)의 시간 경과를 연구하고 특성화하는 학문입니다. 항체, 세포독성 약물(payload) 및 특수 화학적 연결체를 결합한 ADC의 복잡성으로 인해 ADC의 약물동태학적 프로파일을 특성화 하기 위해서는 여러 분석 대상 물질을 측정해야 합니다.

• Total antibody: 약물-항체 비율(DAR)이 0 이상인 ADC

• Drug-conjugated antibody: DAR이 1 이상인 ADC

• free drugs (payloads) or their metabolites

ELISA immunoassay는 총 항체 및 약물 접합 항체의 동역학적 프로파일을 측정하는 데 일반적으로 사용되는 정량 분석 방법입니다. 그림 1에 나타낸 바와 같이 다양한 형식을 적용하여 목표 분석 물질을 검출할 수 있습니다.

Figure 1. Schematic images of ELISA-based ADC PK study

[Case Study]

• Drug-conjugated Antibody measurement by anti-payload antibody and anti-idiotype antibody

Figure 2. Standard curve of DXd conjugated Trastuzumab Sandwich ELISA by anti-idiotype antibody and DXd Antibody. Based on drug-conjugated antibody assay format 2 in Figure 1.

The Sandwich ELISA assay was developed using Anti-Trastuzumab Antibody (15H2), mAb, Mouse (GenScript, A02033) and DXd Antibody (39F4), mAb, Mouse (GenScript, A02217) and as the capture and detection antibodies, respectively.

In this ELISA assay, DXd Antibody (39F4), mAb, Mouse (GenScript, A02217) was labeled with HRP. GenScript can provide customized conjugation service for this product per customer's request.

Capture antibody: Anti-Trastuzumab Antibody (15H2), mAb, Mouse, 2 μg/ml

Detection antibody: DXd Antibody (39F4), mAb, Mouse, 0.22 μg/ml.

• Drug-conjugated Antibody measurement by anti-payload antibody and anti-Human IgG Fc antibody

Figure 3. Standard curve of DXd conjugated Trastuzumab Sandwich ELISA by anti-Human IgG Fc antibody and DXd antibody in monkey serum. Based on drug-conjugated antibody assay format 3 in Figure 1.

The DXd conjugated Trastuzumab Sandwich ELISA assay was developed using DXd Antibody (39F4), mAb, Mouse (GenScript, A02217) and Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb (GenScript, A01854) as the capture and detection antibodies, respectively.

The samples tested in this assay were DXd conjugated Trastuzumab. The control samples were not serum spiked. The monkey serum samples were spiked with cynomolgus monkey serum matrix.

• Total Antibody measurement by anti-idiotype antibody pairs

Figure 4. Standard curve of Trastuzumab Sandwich ELISA. The Trastuzumab Sandwich ELISA assay was developed using Anti-Trastuzumab Antibody (11C4), mAb, Mouse (GenScript, A02032-40) and Anti-Trastuzumab Antibody (15H2), mAb, Mouse (GenScript, A02033-40) as the capture and detection antibodies, respectively. Based on total antibody assay format 4 in Figure 1..

In this ELISA assay, Anti-Trastuzumab Antibody (15H2), mAb, Mouse (GenScript, A02033-40) was labeled with Biotin.

GenScript can provide customized conjugation services for this product per the customer's request. The sensitivity of detecting Trastuzumab was observed to be up to 61 pg/ml.

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