Adeno-associated virus (AAV) vectors are safe and useful gene delivery tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments, both in vitro and in vivo. It has low immunogenicity, is easy to use, and can be safely handled at the lowest biosafety protection levels.

We offer 1,091 in-stock viral vector preps, delivering high quality and ready availability at affordable prices. While our repository serves the needs of many researchers, scientists sometimes require preps beyond our current in-stock capabilities. To meet that need, our Packaged on Request service lets scientists choose from over 5,000 transfer plasmids to be packaged in their choice of nine serotypes, resulting in over 45,000 possible choices!

By using AAV Packaged on Request, scientists can start experiments as soon as they receive their AAV prep from Addgene — eliminating the steps to produce, purify, and titer viral vectors.

Overview

Packaged on Request service는 연구용으로 권장됩니다. 

저장소에 있는 다양한 화학유전학, 광유전학, 재조합효소 및 바이오센서 전달 Plasmid로 이 서비스를 이용할 수 있습니다.

Addgene 저장소에서 원하는 9가지 serotypes과 4가지 volumes의 Plasmid 를 선택하세요.

Details

Scale and Titer

Packaged on Request can be ordered in four sizes. 

*AAV2 yields are generally lower, with a minimum titer of 2 × 1012 GC/mL and a typical titer of 7 × 1012 GC/mL.

Serotype

Packaged on Request can be ordered in nine serotypes:

        • AAV1     • AAV2      • AAV5      • AAV6      • AAV8

        • AAV9     • AAV11    • AAVrg    • PHP.eB

The price for our Packaged on Request preps is all-inclusive of several important steps :

MTA facilitation for the transfer plasmid, including legal approval and an implementation letter

DNA amplification

Viral vector production

Density centrifugation purification

Addgene's comprehensive quality control process:

Titering

Purity assessment

Sterility testing

Viral genome sequencing (VGS)

Request and Ordering Process

AAV 제작에는 약 6~8주가 소요됩니다. 

요청 승인, 소속 기관의 MTA 승인, 배송 등 추가 절차를 포함하면 실제 소요 기간은 요청부터 배송까지 최대 10주까지 걸릴 수 있습니다.